Black Ant (Camponotus Pennsylvanicus)

September 11, 2025

Sample information
Picture
Photos by: Elsa, J
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

black ant found near tree bottom
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

Used standard Taq polymerase. Three cycles.

  • Denaturation (Cycle): 94–98°C for 15–30 seconds.
  • Annealing (Cycle): 50–65°C for 20–40 seconds.
  • Extension (Cycle): 68–72°C 

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My controls worked, and the lines are clear, so I have high confidence
Wolbachia 16S sequence
NNNNNGGNNNNNNNNNGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA ACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT GATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTATAATGGGCTGCAAAGTCGCGA GGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTA ATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
NNNNNNNNNNTTTGCTATTTGATCGGGATAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGA TTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATAC CTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAAT AACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGG TTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATA TTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTA GATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGC TATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGATCCCTCGGGAGGAGGAGATCCTATTTTATACC AACATTTATTTTGATTTTNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus was found to be postive for Wolbachia.
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