Sample information

Hairy, hides in shadows, small

Methods

1. we used a DNA extraction protocol based on the insect adaption of New England Biolabs’ Monarch Spin gDNA Extraction kit

2. the specimen was incubated for 30 minutes in a hot water bath at 56 degrees C

3. Details that differed from the written protocol were in step 16 when liquids were uneven

LAB 7

1. Our first PCR reaction was set up on 10/15/2025

• was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together.
• used an annealing temperature of 49 degrees Celsius
• used this raw polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mic with Standard Buffer (#M0488S)
• no mistakes, time differences, etc

LAB 8

• Our first Gel image was taken in 10/22/2025
• Ran at 125 volts for 23 minutes
• Used this DNA ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains

Lab 9 10/28

– Our second PCR reaction was set up on 10/29/2025, used the same Taq polymerase as the fist PCR reaction and:

• Was a single reaction thwt used the arthropod CO1 primer
• Used an annealing temperature of 49 degrees celsius

Lab 10

• gel electrophoresis system: edvotek gel electrophoresis
• buffer: 1X TAE
• DNA stain: SYBR Safe
• our second gel image was taken on 11/05/2025
• ran at 125 volts for 23 minutes
• used this DNA ladder: new england biolabs 1kb plus DNA ladder for safe stains

Results

all 4 controls match my expectations, the 2nd lane had an error with the DNA process as it was not fully extracted causing no DNA to show up in the lane.

lab 10

• wolbachia is unknown
• confidence level is low
• all 4 controls matched my expectations so for this my confidence level is high but for lane 2, 2-MW, I believe the DNA was not fully extracted so with that hat information i am not confident about my results.