BCC Bumble Bee

October 2, 2025

Sample information
Picture
Photos by: Victor X. Reyes Pagan
Location
Collection date 09/26/2025
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

I found the bee pollinating on campus, and I threw a ziplock bag over it. I caught it at 10:25 am, and kept it preserved in alcohol. for 5 days. On day 6; I brought it to lab.
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus Bombus impatiens

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We may have had a pipette error, so we will try again to get a more accurate reading.

Because I am not able to create a photo with description, I will write down what each lane is.

Lane#1= DNA Ladder

Lane#2= AT

Lane#3= VXRP

Lane#4= +Arthropod Control

Lane#5= -Arthropod Control

Lane#6= Water

Lane#7= +DNA Control

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident in my reading because the specimen I caught will more than likely have Wolbachia. My confidence level is around 8/10 rating.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Bombus impatiens was found to be postive for Wolbachia.
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