Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/01/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | Arthropod was located in the soil underneath some stacked wood and was very active with several others alike; it was approximately 5pm in the afternoon and 63 degrees Fahrenheit outside on a sunny and clear day. |
| Putative identification | Arthropoda Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch DNA Extraction kit (Product # T3010).
|
Results |
|
| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid