Sample information

Orange-red lady beetle with black spots. To short, straight antennae. 6 legs. 2 wings. Circular abdomen. Small head.

Found inside the school.

Late summer

Temperature: 76º F

Habitat: urban

Methods

DNA extraction: Used the whole arthropod. Everything went as planned.

PCR: We added two times the listed amount of ATL buffer and 10 extra microliters of Proteinase K. We had to split the sample into two tubes during DNA extraction and respin them several times. We recombined the tubes at the beginning of the PCR lab.

Gel electrophoresis, Arthropod CO1: in lane 1 was the DNA ladder. C-1 was in lane 2, C-2 was in lane 3, C-3 was in lane 4, C-4 was in lane 5, W+ was in lane 6, W- was in lane 7, H2O control was in lane 8. No mistakes were made. Controls worked.

Gel electrophoresis Wolbachia: DNA ladder in lane 1, C-1 in lane 2, C-2 in lane 3, C-3 in lane 4, C-4 in lane 5, W+ was in lane 6, W- was in lane 7, H2O control was in lane 8. No mistakes were made. Controls worked.

Results

The controls worked, there was no contamination, and the lines showed up strong.