Sample information

I caught this spotted lantern fly at the Rockville metro station. There was several of them crowded along the walkway and I used my glasses case to capture it. The temperature was 67 degrees F. This is a terrestrial organism and hangout out all over city areas. Color Black, Grey, Red, White, Brown, 6 legs, 2 pairs of wings, No antennae, triangular head shape, oval like body.

Methods

PCR

DNA was not properly included during this process. This resulted in a blank gel electrophoresis.

Gel electrophoresis, Arthropod CO1

Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, these were the only two that did not show up well. I believe the gel electrophoresis worked properly because it reflected a previous mistake during the PCR process. DNA wasn’t properly included resulting in no results during gel electrophoresis. Everything else worked well.

Gel electrophoresis, Wolbachia

Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, lane 3 contained A-3, lane 4 contained A-4, lane 5 contained Wolbachia positive DNA, lane 6 contained Wolbachia negative DNA, lane 7 contained water. Each control worked and outside of A-1 and A-2, no mistakes appeared to have been made.

Results

I a confident that this arthropod is does not have Wolbachia. My thought process being that this PCR along with A-1 were repeated due to a mistake. Since two processes were made to determine they were negative that boosts my confidence further