Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | I found the cricket next to a pine tree. There were a lot of crickets around. The cricket had 2 antennas, 6 legs, 2 palps, 13 sections, and 3 tails. It was about 70 degrees outside. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The Gel for the wolbachia part did not have to correct wells. The pellet was a clear white with a gray tint. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod controls not sure about the bands in wolbachia positive and arthropod negative band wolbachia controls- not sure about negative control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be negative for Wolbachia. |
Water Boatman