|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
I found the cricket next to a pine tree. There were a lot of crickets around. The cricket had 2 antennas, 6 legs, 2 palps, 13 sections, and 3 tails. It was about 70 degrees outside.
|Putative identification||Arthropoda Hexapoda Insecta Orthoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
The Gel for the wolbachia part did not have to correct wells. The pellet was a clear white with a gray tint.
|Explanation of confidence level||
Arthropod controls not sure about the bands in wolbachia positive and arthropod negative band
wolbachia controls- not sure about negative control.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Orthoptera was found to be negative for Wolbachia.|