Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/08/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | I found the ant in the crack of a sidewalk near the street. I collected it in the afternoon, at about 7 p.m. there was a few other ants crawling with it. It was warm outside and a bit cloudy |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The buffer with dna was clearly seperated from the cell debris and many off the legs and the exoskeleton was very visible at the bottom also. The pellet was white colored and was visible as specks on the side of the tube. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod controls-not sure about the bands in wolbachia DNA and negative athropod wolbachia controls-not sure about athropod negative band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman