Ant

May 4, 2021

Sample information
Picture
Location
Collection date 04/08/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

I found the ant in the crack of a sidewalk near the street. I collected it in the afternoon, at about 7 p.m. there was a few other ants crawling with it. It was warm outside and a bit cloudy
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The buffer with dna was clearly seperated from the cell debris and many off the legs and the exoskeleton was very visible at the bottom also. The pellet was white colored and was visible as specks on the side of the tube.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod controls-not sure about the bands in wolbachia DNA and negative athropod

wolbachia controls-not sure about athropod negative band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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