Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | This roly-poly was found under backyard flower pot with several roly-polys found in same area. The environment was dark and damp with the temperature at 50-40 degree fahrenheit in afternoon. The insect had segmentation, no wings, dark brown/tan color, a stripe down the middle back, a spotted stripe along sides of back, and aproximately 9 legs were counted. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Throughout the experiment, the gel wells weren’t done correctly, for wells weren’t deep enough. Pipetting may also have had errors, for some may have punctured the wells. DNA may have been accidently mixed slightly when held as well. The pellet of my insect appeared as a visible dark brown. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod controls: not sure about wolbachia DNA bands and negative arthropod Wolbachia controls: not sure about arthropod negative band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman