Roly-Poly

Sample information
Picture
Location
Collection date	04/01/2021
Captive / Cultivated?	Wild-caught
Group	Hampton Roads Academy
Observations	This roly-poly was found under backyard flower pot with several roly-polys found in same area. The environment was dark and damp with the temperature at 50-40 degree fahrenheit in afternoon. The insect had segmentation, no wings, dark brown/tan color, a stripe down the middle back, a spotted stripe along sides of back, and aproximately 9 legs were counted.
Putative identification	Arthropoda Crustacea Malacostraca Isopoda
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	Throughout the experiment, the gel wells weren’t done correctly, for wells weren’t deep enough. Pipetting may also have had errors, for some may have punctured the wells. DNA may have been accidently mixed slightly when held as well. The pellet of my insect appeared as a visible dark brown.
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Arthropod controls: not sure about wolbachia DNA bands and negative arthropod Wolbachia controls: not sure about arthropod negative band
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Isopoda was found to be postive for Wolbachia.

The Wolbachia Project Database