Ant

October 27, 2025

Sample information
Picture
Entry by: Olivia F.
Location
Collection date 09/17/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Found on a wall by other ants. Black. Bigger than a grain of rice.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Methods:

  1. sample prep
  2. cell lysis + DNA precipitation
  3. DNA purification
  4. DNA elution

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Very bright gel line
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Formica was found to be postive for Wolbachia.
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