Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Black ant, a bit larger than a grain of rice. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Formica Formica glacialis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | Lanes 8 and 9 |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | There was a band for Wolbachia DNA and Arthropod DNA |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Formica glacialis was found to be postive for Wolbachia. |
European Paper Wasp
Woodworm Ant