Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/24/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | The spider was outside on a web in the corner of a sidewalk. It looked white to the naked eye but under a microscope it was more brown-looking. It appears to be a garden ghost spider, also known as Hibana gracilis. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | My arthropod ended up having no base pairs, meaning there was no DNA, so something went wrong in the storing process? But other than that, everything else came out to plan, everything looked good! |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid