Sample information |
|
Picture |
|
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Location | |
Collection date | 03/19/2021 |
Captive / Cultivated? | Wild-caught |
Group | Hampton Roads Academy |
Observations | |
Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
Extraction kit | Edwards Buffer |
DNA extraction location | |
Single or Duplex PCR | |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | First time doing this experiment, and the control worked. My arthropod did not have wolbachia. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Hymenoptera was found to be negative for Wolbachia. |