Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | The arthropod is similar to an ant, but longer, has a black body, yellow legs, no wings, antennae, and a sectioned rear. |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | I crushed my pellet firstly, which took a amount of time though it seemed to eventually settle in the bottom of the tube. My pellet was very dark and very compacted. Most of the experiment went as planned, though their was one hiccup on our part described in the confidence level explanation. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There was a possible miscalculation in the cell lysis step. The DNA could have been diluted. The pellet was very dark, and very compacted. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Blattodea was found to be postive for Wolbachia. |
Water Boatman