Termite

May 1, 2021

Sample information
Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The arthropod is similar to an ant, but longer, has a black body, yellow legs, no wings, antennae, and a sectioned rear.
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I crushed my pellet firstly, which took a amount of time though it seemed to eventually settle in the bottom of the tube. My pellet was very dark and very compacted. Most of the experiment went as planned, though their was one hiccup on our part described in the confidence level explanation.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There was a possible miscalculation in the cell lysis step. The DNA could have been diluted. The pellet was very dark, and very compacted.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be postive for Wolbachia.
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