Ant

April 29, 2021

Sample information
Picture
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The ants were walking in a line to and from the large azalea bush, which has the nest in it. They used a crack in the driveway for shelter.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was not visible

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There was a band for Wolbachia, but it was very faint.

Arthropod control -A didn’t seem to work

-A, +A control in Wolbachia didn’t seem to work.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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