Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | The ants were walking in a line to and from the large azalea bush, which has the nest in it. They used a crack in the driveway for shelter. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The pellet was not visible |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There was a band for Wolbachia, but it was very faint. Arthropod control -A didn’t seem to work -A, +A control in Wolbachia didn’t seem to work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman