Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/10/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | This specimen is yellow-brown in color, legs thicker toward the abdomen and slims down toward the foot and is fast-moving when uncovered. |
| Putative identification | Arthropoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | EFSA2 Lithobius forficatus had DNA band highly visible around 708 base pairs in gel electrophoresis (which is known size of CO1 barcoding gene) in Lane 4. When run through gel electrophoresis, for Wolbachia, EFSA2 presented no DNA bands (negative) around 438 base pairs (which is the known size of the 16s rRNA) in Lane 4. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Was not sent for Wolbachia sequencing. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Chilopoda was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR