Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/28/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | My ant was located on a window seek near house plants. It had 2 antennas, 6 legs, and no wings. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was small and white. When using the pipet to take out part of the supernatant, I disturbed the pellet. I had to redo this step. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low because I cannot see a precise band. It appears to be more of a smear. The band was faint. First time doing the experiment, not sure about the +A band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman