Sample information |
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Picture |
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Location | |
Collection date | 03/03/2021 |
Captive / Cultivated? | Wild-caught |
Group | Hampton Roads Academy |
Observations | This ant was caught in a backyard. It took a while because I was trying to not destroy the arthropod. In addition, under the when microscope, the ant was a dark brown color and its legs were weirdly bent. |
Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
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Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | During my experiment, much of the lysis buffer spilled out onto the sides. In addition, the pellet was disturbed twice. The first time, I did the correct procedure and repeated the required steps. The second time, due to management, I was not able to and continued the experiment. My pellet was barely visible to the human eye and could only be seen when held up to the light. |
Results |
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Wolbachia presence | Yes |
Confidence level | Medium |
Explanation of confidence level | My confidence was medium because there was clearly a Wolbachia band, but due to my inexperience, there is no way to be sure about this. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Hymenoptera was found to be postive for Wolbachia. |