Sample information

Collection date 03/03/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

This ant was caught in a backyard. It took a while because I was trying to not destroy the arthropod. In addition, under the when microscope, the ant was a dark brown color and its legs were weirdly bent.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

During my experiment, much of the lysis buffer spilled out onto the sides. In addition, the pellet was disturbed twice. The first time, I did the correct procedure and repeated the required steps. The second time, due to management, I was not able to and continued the experiment. My pellet was barely visible to the human eye and could only be seen when held up to the light.


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence was medium because there was clearly a Wolbachia band, but due to my inexperience, there is no way to be sure about this.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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