|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
This ant was caught in a backyard. It took a while because I was trying to not destroy the arthropod. In addition, under the when microscope, the ant was a dark brown color and its legs were weirdly bent.
|Putative identification||Hexapoda Insecta Hymenoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
During my experiment, much of the lysis buffer spilled out onto the sides. In addition, the pellet was disturbed twice. The first time, I did the correct procedure and repeated the required steps. The second time, due to management, I was not able to and continued the experiment. My pellet was barely visible to the human eye and could only be seen when held up to the light.
|Explanation of confidence level||
My confidence was medium because there was clearly a Wolbachia band, but due to my inexperience, there is no way to be sure about this.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hymenoptera was found to be postive for Wolbachia.|