Lady Bug

November 13, 2025

Sample information
Picture
Entry by: Tanashri G.
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations
  • Found on the ceiling walking near a light stimuli (lamp)
  • Energetic
  • Flys around
  • Dark red with small circular black spots
  • Oval shaped
  • Six legs
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Our group worked productively with our time and used precise measures when working with all the samples and parts of the experiment.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The bands from the gel electrophoresis and the quality score display that the ladybug was Wolbachia negative, therefore a high confidence level that the ladybug is not positive.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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