Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/07/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Location Description: Pine straw outside of school underneath in moist soil. Not much light exposure and surrounded with pill bugs Rounded back with shelled exoskeleton, red and coloration, many limbs estimated around 50 legs, small antenna, long in length.
|
| Putative identification | Arthropoda Diplopoda Polydesmida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I have low confidence in these results because during the DNA extraction process, the specimen wasn’t preserved at the right time and was put into the alcohol after it had already been dried up. This most likely interfered with the results regarding the Arthropod Data. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Polydesmida was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR