Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | This Damselfly was found in the tall grass around Lake Saint Benedict. We used the sweep net and swept it through the tall grass. |
| Putative identification | Arthropoda Insecta Odonata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My Arthropod DNA was heavily contaminated which essentially made it unusable. I believe that i could have made an error during the PCR step. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Differential Grasshopper – Melanoplus differentialis
Pill Bug (Armadillidium vulgare) – Draft
Melanoplus Femurrubrum
Grasshopper – Orthoptera
Cisseps Fulvicollis