Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | The insect has a distinctly bicolored body: the head and middle section (thorax) are reddish-brown, while the rear section (abdomen) is shiny and dark brown or black.
|
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I had DNA for the arthropod, but no Wolbachia on the gel band |
| Wolbachia 16S sequence |
no wolbachia found
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Cisseps Fulvicollis
Aboud kanama
(no title)
Blaesoxipha sarcophagidae