Mosquito – Anopheles gambiae

December 9, 2025

Sample information
Picture
Photos by: Aisha Alarmouty
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students. Male mosquito with plumose (feathery) antennae. collected behind the Goodwin Hall building at the Benedictine University campus.attachments

Putative identification Arthropoda Insecta Diptera Culicidae Anopheles Anopheles gambiae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My insect BLAST match was 100. Clear arthropod sequence 100% ID. No Wolbachia band detected.
Wolbachia 16S sequence Download FASTA   
TGCAGGGTCAAAGAAAGAAGTTTCGGTCAGTTAAAAGTATAGTAATAGCTCCGGCTAATACAGGTAGGGATAAAAGAAGT AAAGTAATAAATACGGATCACACAAATAAAGGTATTTTATCACTCGTATATTAATAATTGTTGAAATAAAATTTACAGCC CCTAAAATTGATGAAATACCTGCAAGATGTAAGGAAAAGATTGAAAGATCTACAGAGGCCCCCGTATGTGCAATAGTTGA AGACAAAGGAGGATAAACTGTTCAACCTGTTCCAGTTCCTCTATCTACCAAACTACTTGTTAATAAAAGAGTTAATGATG GAGGTAATAACCAAAATCTTATATTATTTAATCGGGGAAATGCTATGTCAGGGGCGGATAATATTAATGGAACTAATCAA TTTCCAAATCCTCCAATTATAATTGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCAGTTACAATTACATTATA AATTTGATCATCTCCAATTAAAGCAAATGGATAACCTAATTCTGCTCGAATTAATATACTTAATGATGTTCCTACTATTC TAGACCACGCCCCAAAAATAAAATATAATGTTCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ACAGTTGTAATTACTACTGATCAAACAAATAATGAAACTCCTGCCAGGTGTAAAGAAAAAATAGCCAAATCTACAGAAGC TCCTCTATGAGCAATTGCTGCTGATAAAGGAGGGTAAACAGTTCAACCTGTTCCTGCACCATTTTCAACAAATGAACTAG AAAGAAGAAGAGTAAGAGAAGGGGGTAAAAGTCAAAAACTTATATTATTTATTCGAGGAAATGCCATATCTGGGGCTCCT AATATTAAAGGAATTAATCAATTACCAAATCCTCCAATTAAAATAGGCATAACTATAAAAAAAATTATAATAAAAGCATG AGCTGTGACAACTACATTATAAATTTGATCATCTCCAATAAATGTACCAGGTCGTCCTAGTTCAGTTCGAATTAATATGC TTAGAGATGTACCAACTATCCCTGATCAAGCTTTGGGGGGGCGCACAAACGCCTAAATTCGGCCACTGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Anopheles gambiae was found to be negative for Wolbachia.
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