Opilio parietinus

December 8, 2025

Sample information
Picture
Photos by: Aisha S.
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

I caught my Arthropod nead some tall grasses by a tree. This also happens to be the type of habitat in which  my arthropod naturally resides.
Putative identification Arthropoda Arachnida Opiliones Phalangiidae Opilio Opilio parietinus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

An error occurred during the step where the master mix was added to the DNA, which affected the samples before they were loaded onto the electrophoresis gel. Adding too much mastermix skewed the results and prevented any Arthropod DNA from showing in the results.I obtained a negative result for my arthropod sample, which was likely due to an error during preparation of the master mix

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I obtained a negative result for my arthropod sample, which was likely due to an error during preparation of the master mix. Despite this, I was still able to get some Wolbachia DNA. In my BLAST results, I had a percent identity of 99.73%.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGAGCTGTTGATCTCATATTGGATTAACGTCGACGCCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCAT GCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCCCGACTTTGCAGCCCATTG TAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTT ATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAA CCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGA ATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Opilio parietinus was found to be postive for Wolbachia.
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