Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/01/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | The insect is shaped like a leaf, which made it stand out from the background, it was found resting on the trunk of the tree. |
| Putative identification | Arthropoda Insecta Hemiptera Fulgoridae |
Methods |
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| Extraction kit | container |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | In lane 3 is my Plant hopper(Fulgoroidea) sample. The gel showed a band for its DNA, indicating it was successful. It also showed a band for Wolbachia DNA , meaning my insect tested positive for Wolbachia. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My blast result showed 97% query cover, and 100% percent identity. It had an E-value of 0.0 for all top matches. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GATGTGCTGTAAATTTCGGTCTATAATTAATATTGTGATTGCTCCGGCTAGTACTGGTAAAGAAACTAGTAAAAGGATTG CTGTAATTAGTACTGATCAACAAAATAAGGGGGTTTTTTCTATTGTTATTCCTTTGATTCGTATATTTATAATGGTTGAA ATAAAATTAATTGCTCCTATAATTGATCTAATACCTGCAATGTGTAATGAAAAAATTGTTAAGTCTACTGATGGTCCTGA ATGTGCTTCTTGAGCCGAAAGAGGTGGGTATACAGTTCATCCTGTTCCTGTTCCTGAAGCTGCTGTTGATCTTGCAATTA ATAATGTAATTGATATTGGTAAAAGTCAAAAACTTATATTGTTTATTCGTGGGAAAGCTATATCTGGTGCTCCAATTATA AGAGGAACAAGCCAGTTTCCAAAACCACCAATTATAATTGGTATAACTATAAAAAAGATTATTACAAATGCATGTGAAGT TACAATTGTGTTATAAATTTGGTCATTTTTGATTATTGATCCTGGTTGAGATAGTTCTATTCGAATAATTATTCTTATTA TTATTCCTACTAGTCCTGATCAAATTCCAAATATAAAATATAGTGTTCCAATATCTTa
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Fulgoridae was found to be postive for Wolbachia. |
Cisseps Fulvicollis
Aboud kanama
(no title)
Blaesoxipha sarcophagidae