Sciara hemerobioides

Sample information

Picture

Location

Collection date

Captive / Cultivated?

Wild-caught

Group

Benedictine University

Observations

The arthropod was collected on the Benedictine University campus near the garden area during the early fall season. It was found resting on a bunch of Queen Annes’s flowers and was collected using a jar and capturing the whole flower. After capture, the insect was placed in a killing jar to secure and preserve the sample. The weather was sunny, and the collection occurred in the afternoon. The insect showed a still reaction when approached, remaining mostly still on the leaf in the open area.

Putative identification

Arthropoda Insecta Diptera

Methods

Extraction kit

Blood and Tissue kit

DNA extraction location

Whole arthropod

Single or Duplex PCR

Single Reaction

Gel electrophoresis system

Standard electrophoresis system

Buffer

TAE

DNA stain

SYBR Safe

Gel images

Protocol notes

Results

Wolbachia presence

Confidence level

Low

Explanation of confidence level

The BLAST results produced a relatively low match, which provides limited support for identification and leaves considerable uncertainty. The controls behaved as expected, confirming that the PCR reagents and conditions were functioning properly. The positive fruit fly control generated a clear Wolbachia band at the correct size, validating the assay. The negative control showed no bands, ruling out contamination. In contrast, the arthropod sample displayed only a faint COI band, indicating that DNA extraction and amplification were weak and that very little insect DNA was present. No Wolbachia band was detected in the sample. Overall, the confidence level is best described as low due to the weak COI signal and poor BLAST alignment.

Wolbachia 16S sequence

Arthropod COI sequence

Summary

The Diptera was found to be negative for Wolbachia.

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