Gryllus sp. (Cricket)

December 9, 2025

Sample information
Picture
Photos by: Victoria Alvarez
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

The insect was dark and long and the abdomen was a very glossy almost black color with a slight hint of green to it.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I had no DNA nor Wolbachia bacteria
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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