DEM-VEF Orthoptera

December 9, 2025

Sample information
Picture
Photos by: Virginia F.
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time: 1:15-1:25 P.M. EST

Location: Field located by Science Center parking lot, approximately 60-70 yards from the front right-hand side of the greenhouse, near a few shrub/tall grass.

Environment: Plain grassy field with muddy, mushy, soil. Few vegetation/shrubs/bushes grouped together, which many insects sought shelter in. Partial clouds. Grass was not long, however some dead grass that covered some areas felt like straw. Very green. Few flowers.

Method: Via sweep net
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Controls as expected. Genomic DNA quality assessed to be reliable. Low probability of sample contamination. Ladder provides accurate band reference. No errors recorded during experimentation process.

Local data specified that this order had ~ 27.5% infection rate. These statistics were gathered from 2013-2015. While less common in Orthoptera than other orders, infection is not rare by any means.
Wolbachia 16S sequence
TATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTcacg ttttCgAgTTGCAGAGTACAATCCGAACTGAGATGTCTTTTGGggatTAG CTTAGGCTTGCGCACCTTGCAACCCATTGTAGACACCATTGTAGCACGTG TGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTC CTCCAGCTTATCACTGGCAGTTTCCTTAAAGTACTCAGCATTACCTGATG GCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTC ACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGA ACCGACCCTATCCCTTCGAATAGGTATGannnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnncttttTTtttttttttttTtttttttttTTTTTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTGGAGCTTGAGCTGGgataGTAGGCACATCATTAAGTATTTTAATTCGA ACAGAACTAGGACAACccgtctATTTAATTGGAGATGATCAAACTTATAA TGTTATCGTAACCGCACATGCATTTATCATAATTTTCTTTATAGTTATAC CTATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTGGGG GCTCCAGATATAGCCTTCCCACGAATAAACAATATAAGATTTTGACTTCT ACCCCCATCATTAACCCTTTTATTAACCAGAAGAATAGTTGAAAATGGTG CAGGAACAGGATGAACAGTATACCCACCTCTATCAACAGGAATCGCTCAC GCGGGAGCATCCGTCGATTTAGCTATCTTCTCACTACATTTAGCAGGAAT TTCCTCAATTCTAGGGGCTGTAAATTTTATCACTACCATAATTAATATAC GAGCTCCAGGAATATCACTAGACCAAACACCATTATTTGTTTGAGCAGTC GGAATTACAGCTCTTCTATTATTATTATCCTTACCAGTTTTAGCTGGTGC TATTACAATATTGCTTACAGATCGAAATTTAAATACATCATTTTTTGATC CAGCGGGAGGAGGGGATCCAATCTTATATCAACATCTATTTTGATTTTTT GGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be postive for Wolbachia.
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