Sample information
Picture	Photos by: Rachel C.
Location
Collection date	09/03/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations
Putative identification	Arthropoda Insecta Orthoptera
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	agarose gel electrophoresis
Buffer	lysis
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1
Summary	The Orthoptera was found to be negative for Wolbachia.