Pill Bug (Armadillidium vulgare) – Draft

December 12, 2025

Sample information
Picture
Photos by: Sara V.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Found in the grass patches near Goodwin and Krasa
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer Other
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I had a faint line at 438bp
Wolbachia 16S sequence
>65-EW_G1_Wo.ab1 (370 bp) CGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAAC TGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCC ACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAG CATTATCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGAC AGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCTTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
>31-EA_G2_CoR.ab1 (587 bp) GTATTTAAATTACGGTCAGTCAATAGTATAGTAATAGCTCCAGCAAGTAACAGGTAAAGATAAGTAGAAGAAGAACCGCG GTTACTAAAACAGACCAAACAAATAAAGGCACACGATCCATACTAATTCCGGACGCCCGTATATTAATTATAGTCGTAAT AAAGTTTACTGCCCCTAAAATTGATGAAGCCCCAGCAAGATGAAGAGAAAAGATACCTAAATCTACAGAAGCTCCACTAT GGGCAATTCTTGATGCCAAAGGAGGGTAAACAGTTCATCCCGTTCCTACTCCTCTTTCAATTAATCCTCTGCTAAGGAGT AAAGTTAAAGAAGGGGGAAGCAGCCAAAATCTTATATTGTTTATACGAGGGAAAGCTATATCAGGAGCCCCTAATATCAA AGGAATTAATCAATTTCCAAAACCTCCAATTATAATAGGTATTACTATGAAGAAAATCATTACAAAAGCATGAGCCGTGA CAATTACATTATAGATTTGATCATCCCCAATGAGACTCCCAGGCTGCCCTAATTCAGTCCGAATAATTATTCTTAAAGCG GTACCTACAGCCCCTGCCCAAACTCCG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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