Cisseps Fulvicollis

December 10, 2025

Sample information
Picture
Photos by: Ronaldo Z
Location No map location data available.
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Was caught using a net and then submerged in an ethanol based solution to preserve DNA. End results showed to have no Wolbachia.
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer lysis
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The DNA concentration results proved to be much higher than the standard amount which may suggest there was either protein or salt contamination present in the DNA sample
Wolbachia 16S sequence Download FASTA   
TATTATGCTGATAGAGATAGGATCTCCCCCTCCTGCTGGATCAAAAAATGAAGTATTTAAATTACGATCAGTTAGTAATATAGTGATTGCACCAGCTAATACAGGTAAAGAAAGTAATAATAAAAATGCTGTAATTCCCACTGCTCAAATAAATAAAGGTATTTGATCAAAGGATAAATTATTTAATCGTATATTAATAATTGTTGTGATAAAATTAATTGCACCTAAGATTGATGAGATTCCTGCTAAATGAAGAGAAAAGATAGCAAGATCTACTGAACTTCCAGCGTGAGCAATATTAGAAGAAAGTGGGGGGTAGACTGTTCATCCTGTTCCTGCTCCATTTTCTACAATTCTACTTGAGATTAATAAAGTGAGAGATGGGGGTAATAATCAGAAGCTTATATTATTTATTCGAGGGAAGGCTATATCAGGTGCTCCTAATATTAAAGGTACTAATCAATTACCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAATTATAATGAAAGCATGAGCTGTGACAATAGTATTATAAATTTGATCATCTCCAATTAAAGATCCAGGATTACCTAATTCAGCTCGAATTAATAATCTTAGAGAAGTTCCTACTATACCTGCTCAAATACCAAAAATAAAATATAATGTTCCAATATCTTTTCCCCACCTATCATCTTCTTTTTTTAAACTGCCTCAGTTGATGATGAAACATTAGTGAGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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