Grasshopper – Orthoptera

December 11, 2025

Sample information
Picture
Photos by: Shayan H.
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected with killing jar in grassy area
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls worked as they should, and I had no band in the Wolbachia region. However, I had a small faint band in the COI region making my confidence medium.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Melanoplus was found to be negative for Wolbachia.
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