Melanoplus Femurrubrum

December 11, 2025

Sample information
Picture
Photos by: Maria Q.
Location
Collection date 09/02/2025
Captive / Cultivated? Captive / Cultivated
Group Benedictine University
Observations

It thrives in disturbed areas like roadsides and fields. Has a red hind for jumping
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit tweezers
DNA extraction location Reproduction
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer lysis
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

100%

  • DNA was contaminated → PCR failed → no gel band
  • Likely due to inaccurate micropipetting (pulled up too much sample)
  • Used morphology + Seek App 
  • Identified arthropod as red-legged grasshopper
  • Plan to improve: pipette more carefully to avoid contamination

 
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A Did not find insect
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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