December 18, 2025

Sample information
Picture
Entry by: Matthew W.
Location
Collection date 11/12/2025
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Small arthropod, possibly Austrosciara hawaiiensis species but still unknown.
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

A well ground pellet was obtained during the PCR. All controls on the control gel were also accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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