Sample information

Tiny grey moth with small wings and a tiny thorax

Methods

Place the gel tray containing your gel in the buffer chamber
Ensure that the clear buffer chamber is inside the blueGel electrophoresis system.

The wells of the gel should be on the same side as the negative electrode, away from the power button.

Use a graduated cylinder to add 30 ml of 1X TBE electrophoresis buffer
The buffer should just cover the gel and wells.

Ensure that there are no air bubbles in the wells (shake the gel gently if bubbles need to be dislodged).

Starting with the ladder in lane 1, gently hover your pipette tip over the well, and load 5 uL. Follow the loading key in the table below to load the rest of your gel. Remember to change tips between each sample.

Place the orange cover on the blueGel electrophoresis system
Match the positive and negative electrode signs on the orange lid with the corresponding positive and negative signs on the blue base.

The orange lid should sit flush with the blue base using little force.

Press the “Run” button
Check that the green light beside the power button remains illuminated.

Conduct electrophoresis for 20-25 minutes
The colored dye should progress to about half the length of the gel.

Longer electrophoresis times will result in better size resolution.

Press the “light bulb” button to turn on the blueGel transilluminator.
For best viewing, place the viewing chamber over the blueGel machine.

Gels may be viewed at the end of the run or periodically throughout the run.

Ensure that the bands in your gel have separated enough to clearly interpret your results
Run the gel longer if needed to increase resolution.

Take a picture and insert it in the correct post-lab question

Results

After reviewing the results from the mini-PCR pcr there was no indication whatsoever that the specimen had the Wolbachia 16S Band