7I #1

January 6, 2026

Sample information
Picture
Entry by: Izzy M
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

It has a head that is relatively separated from the abdomen. There are six legs and it has antennae. The thorax is thin.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain Cyber Green
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The confidence level is high because the controls were correct, there was no bleed whatsoever, and there was a very clear line indicating that arthropod DNA was present, but not Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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