Sample information
Picture	Entry by: Cadence P
Location
Collection date	09/05/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	It has a black, tough exoskeleton and light brown legs. Also has six legs and two antennae.
Putative identification	Arthropoda Insecta Coleoptera
Methods
Extraction kit	Qiagen Blood and Tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	agarose gel electrophoresis
Buffer
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coleoptera was found to be negative for Wolbachia.