Coleoptera

February 16, 2026

Sample information
Picture
Entry by: Gwen U
Location
Collection date 09/06/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found the specimen crossing the sidewalk. I collected it mid-day, around 12 pm. It was around 60 degrees, slightly windy, and cloudy. I attached a picture of the area where I collected the specimen. There were not many other bugs out when I collected it.
Putative identification Arthropoda Insecta Coleoptera Carabidae Harpalus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TBE
DNA stain SeaGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

DNA could be seen in the gel.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Harpalus was found to be negative for Wolbachia.
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