Areneus Diadematus W-negative

January 14, 2026

Sample information
Picture
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I caught this spider in the bushes outside in the lunch area at my school. I remember the spider being about the length from the tip of my thumb to the first joint counting its legs, although I may be remembering it bigger than it was. It was a sunny day in September if I recall correctly, and it was early in the afternoon.
Putative identification Arthropoda Arachnida Araneae Araneidae Araneus Araneus diadematus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My instructor and I suspect that the streak for the wolbachia lane was caused by contamination.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Araneus diadematus was found to be negative for Wolbachia.
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