Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State University |
| Observations | The arthropod was found by itself along a path through Hort Woods. It was found at 6:11 pm on September 29, 2025. The weather was about 72 degrees and cloudy. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus |
Methods |
|
| Extraction kit | QIAGEN DNeasy kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | The gel shows many filled lanes due to an error during well filling. Each sample was pipetted into two wells instead of one. Gel electrophoresis lanes: 1- ladder 2,3- lanes 2 and 3 were the arthropod sample
|
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The controls worked, and there were no issues with the protocol besides the extra wells being filled. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Camponotus pennsylvanicus was found to be postive for Wolbachia. |
(no title)
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