American Winter Ant

January 29, 2026

Sample information
Picture
Entry by: Harrison M
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

2.5 – 4.5 mm long, black abdomen and head, dark brown midsection, brown legs and antenna
Putative identification Arthropoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TBG
DNA stain SYBR Safe
Gel images
Protocol notes

Standard

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The PCR reaction likely failed, as demonstrated by the lack of bands in the controls. This means that there can be no certainty in the results of the reaction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Prenolepis imparis was found to be negative for Wolbachia.
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