Wolbachia Larger than Life

May 13, 2026

Sample information
Picture
Entry by: Nora H
Location
Collection date 02/16/2026
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Was found near water source indoors.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Tapinoma Tapinoma sessile

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We were given protocol of 30 to 60 minutes for incubation and only used 50 minutes given time limit.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

After duplex PCR was determined to have a positive result I then ran a single PCR and got the same results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tapinoma sessile was found to be postive for Wolbachia.
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