Lacewing GRP14

Sample information
Picture	Entry by: Kaylynn S.
Location
Collection date	04/29/2026
Captive / Cultivated?	Wild-caught
Group	California Academy of Mathematics and Science
Observations	Sighted under a leaf of a pomegranate tree, where there was a cocoon, and seemed attracted to the flashlight.
Putative identification	Arthropoda Insecta Neuroptera Chrysopidae
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	agarose gel electrophoresis
Buffer	1X TAE
DNA stain	UView
Gel images
Protocol notes	DNA Extraction Protocol Gel Electrophoresis Protocol
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	Based on the reuslts from gel electrophoresis, once put on a UV lightbox our ladder appeared and there was one band present on the lane in which we loaded the lacewing sample. That one band had the same amount of base pairs an arthropod would have, which was obtained by the ladder. So, due to this we concluded that it was an arthropod and not wolbachia infected.
Wolbachia 16S sequence	Download FASTA
Arthropod COI sequence	Download FASTA Download AB1 5' GCCCTTAGAATAATTATTCGCACTGATTTTTTGGTCACCCTGAAGTTTAA 3' BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Chrysopidae was found to be negative for Wolbachia.

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