Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 12/12/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | Spider found dead in corner of patio in web. Found & collected at about 12:00am. Temp reports to be around 36°F. No other spiders or webs in the area. Spider about 3-4 mm in length. Spider is light brown with large pedipalps. Spider is not hairy, but not smooth. Appearance matches Theridiidae spiders. |
| Putative identification | Arthropoda Arachnida Araneae Theridiidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Arthropod gel shows band in all lanes excluding water, so we know PCR worked. Wolbachia controls showed expected results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Theridiidae was found to be negative for Wolbachia. |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly