Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 12/06/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | During the time I captured the Cricket, I believe it was late in the day, around 6 P.M. It was found on the sidewalk, near my front yard. Since it was found during the early days of December, I believe that it was around 40-50°F. Additionally, it has antennae, 6 legs, and 3 body segments. |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes |
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Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Throughout this entire process, I had much confidence in our experiment. However, I was sadly proven wrong when it came to our Wolbachia results. I believe that we did everything correctly. Our PCR, Electrophoresis, and DNA Extraction, went smoothly, so seeing these unexpected result was a shocker to us. The bands did show where we expected them to in our arthropod gel. Unfortunately, only the DNA Ladder appeared in our wolbachia gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly