Beatle Larva

July 16, 2021

Sample information
Picture
Photos by: Aubrey R.
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Has a black face, white butt, grey scale like skin, six legs, and an orange bump above its head.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The bug is in the fourth well. I extracted the DNA by crushing the whole bug in the tube.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My control didn’t show up so I don’t know if there is any or not.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phyllophaga was found to be negative for Wolbachia.
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