Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/06/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | Very Small ant found under log legs red and the rest is black has five legs one antenna is longer then the other tip of antenna is a light red tip of the legs is light red the back half is blacker then the head and body |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | I put it though many machines |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | There were no lines on the gel |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |


Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid