Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/26/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | Ant with no special observations. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | Cyber Green |
| Gel images |
|
| Protocol notes | column 2) Is the examined ant, it shows a light strip of Wolbachia DNA and a strong Insect DNA.->ant is positive Wolbachia 3) (Not visible) is the positive test. 4) Is the negative test 5) Wolbachia example 6) water 7) DNA-ladder |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | The postive test didn`t work properly. And the DNA-ladder are extremely strong. Also the Wolbachia strip is not significantly coulored and doubled. The negative control showed a positive result. Therefore the test isn`t significant.
negative control- insect without wolbachia positive control- insect with Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR