BRI-AMM Hemiptera

April 16, 2026

Sample information
Picture
Entry by: AM
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insects were very few because of the season. Mine was tiny, round, and off-white with red eyes.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Larva
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I made several mistakes along the way, but my results matched my predictions.
Wolbachia 16S sequence
TATGATCAGGACTAATAGGAACAATAAGAAGAATAATCATCCGAATAGAACTAATTCAACcagtatCAATTATTAACAACGACCAAATCTACAATGTAATAGTTACTTCACATGCATTCATCATAATTTTTTTCATAGTTATACCAATCATAATTGGCGGATTCGGAAACTGATTAGTTCCAATTATGATTGGAGCTCCAGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACTCCTTCCCCCATCATTAACACTAATAATTTCAAGAAGATTAACAGGAACAGGCTCAGGAACAGGTTGAACAGTTTATCCTCCATTAGCAAGATTAACAGCTCATTCAGGCCCATCAGTAGACTTAACAATTTTCTCACTTCATATTGCAGGAATTAGATCTATTTTAGGAGCAATTAACTTTATCTCAACCATCATAAACATACGATCAGAAGAAATATCACCAGAAAAAACCCCCCTATTCTGCTGATCAGTATTAATCACAGCAATTCTCCTCCTAATCTCACTACCAGTGTTAGCAGGAGCAATTACTATATTACTAACAGACCGAAATTTTAATACATCATTTTTTGACCCAACCGGAGGAGGAGACCCAATCTTATACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GTGTACAGacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCATTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATCATCCCCATCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAGCATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACAC=CTGTGTAAAATCCGGCCGAACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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