Ant – 2 BASD

Sample information

Picture
Entry by: Kaylee S
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

The temperature was around 64 degrees and this ant was collected in a small forrest area.

Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

DNA Extraction: the whole ant was used.

1.)  Ladder

2.) Insect Sample #1 (bee)

3.) Insect Sample #2 (ant)

4.) + Fruit Fly

5.) – Fruit Fly

6.) + DNA

7.) – – water

Analysis: my controls worked. Lane six did spill over to lane seven though, so for the water lane we had to use lane eight. That would be why lane six and seven has a one big strand.

Results

Wolbachia presence
Confidence level
Explanation of confidence level

For the Wholbachia gel, only the positive Wholbachia strand band was the only one that appeared. It makes me wonder if something went wrong, or if this could be an expected result. We also got no results on the positive DNA 6th lane which makes me more curious as to what could have happened.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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